ABSTRACT
Aims: To ascertain the antidiabetic and antioxidative effects of ethanol-methanol extracts of leaf, stem bark and root of Jatropha curcas on the blood and liver tissue of streptozotocin-induced diabetic albino wistar rats Place and Duration of Study: Department of Medical Biochemistry, Cross River University of Technology, Okuku campus between August 2013 and March, 2014. Methodology: Fifty four (54) male albino wistar rats weighing 150- 200g were randomly assigned into nine study groups (n=6). Group I was the normal control, groups II–VI were induced with diabetes using streptozotocin. Group II was untreated, while groups III –VI were treated with leaf, stem bark, root extracts and Glibenclamide (standard drug) respectively. The remaining groups VIIIX were not induced with diabetes but were treated with leaf, stem bark and root extracts respectively. The administration of these extracts lasted for 14 days after which the animals were sacrificed. The liver tissue was collected and homogenized and the supernatant used for the estimation of SOD, CAT and MDA activities. Results: The result of blood glucose level on the fourteenth day of the plant extracts administration, showed further decrease in the groups treated with the plant extracts to the extent that group IV was significantly (P<0.05) decreased compared with the normal control. The blood glucose level of the standard drug (Glibenclamide) treated group (VI) was further decreased however it remained significantly (P<0.05) increased compared with the normal control and about three or more times that of the groups treated with the plant extracts. Result of the liver tissue SOD for the test groups showed significant (P<0.05) difference except for groups IV and VII when compared with the normal control. There was no significant (P≥0.05) difference in the SOD activities of test groups compared with the diabetic control. The liver tissue CAT showed general increase for the test groups, but group III produced a significant (P<0.05) increase compared with the normal control, while groups III and V showed significant (P<0.05) increase compared with the diabetic control. The liver tissue MDA showed significant (P<0.05) increase for groups II and IV but a significant (P<0.05) decrease for group V compared with the normal control. However, compared with group II (diabetic control) all the test groups showed a significant (P<0.05) decrease. Conclusion: The present research suggest that the leaf, stem bark and root of ethanol-methanol (1:1) extracts of Jatropha curcas possess anti-hyperglycemic and antioxidant activities but their response to the liver tissue enzyme systems of SOD and CAT vary in either to playing compensatory role or boosting the activities of the antioxidant enzymes. The plant parts also appear to possess the potential for reversing the tissue oxidative damage caused by diabetes as seen by their abilities to prevent lipid peroxidation.
ABSTRACT
The effects of various extracts of Ocimum basilicum leaf on biochemical indices of organ damage and oxidative stress status of streptozotocin-induced diabetic rats were examined. Oral administration of 200mg/kg of aqueous, methanolic and petroleum ether extracts of the leaf for 35 days resulted in a significant (P<0.05) reduction in thiobarbituric acid reactive substances (TBARS) and an increase in catalase (CAT) and superoxide dismutase (SOD) activities in streptozotocin-induced diabetic rats from diabetic levels. The leaf extracts brought about a significant (P>0.05) increase in serum protein and albumin as well as decreases in urea and creatinine levels of STZ – induced diabetic rats compared with diabetic control levels. The Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) levels increased significantly (P>0.05) in diabetic control group. The extracts caused a significant reduction in levels of AST and ALT in treated diabetic groups and maintained the normal level observed in normal rats. In this study a significant decrease in PCV was observed in diabetic control group and increase in the PCV in rats given extracts. It was concluded that the extracts have in vivo antioxidant, hepatoprotective and nephroprotective effects in STZ – induced diabetic rats. These results support its traditional use in the management of diabetes and cardiovascular diseases.
ABSTRACT
The antiplasmodial activity of the aqueous leaf extract of Mucuna pruriens (M. pruriens) was evaluated against Plasmodium berghei NK-65 strain in mice. The plant was selected based on their traditional claims for treatment of fever and other malaria related diseases in southeastern region of Nigeria. An aqueous leaf extract (90 – 270 mg/kg) was investigated for antiplasmodial activity against Chloroquine-sensitive Plasmodium berghei infections in mice. The antiplasmodial activity during early and established infections as well as prophylactic action of the plant in blood was investigated. Chloroquine (10 mg/kg) and pyrimethamine (1.2 mg/kg) were used as positive controls. The extract (90 – 270 mg/kg) dose dependently reduced parasitaemia induced by Chloroquine sensitive Plasmodium berghei infection in suppressive, prophylactic and curative models in mice. The extract at these doses caused 60.06 – 71.75% inhibition of parasitaemia in the suppressive test, 65.97 – 84.38% parasitaemia inhibition in prophylactic test and a mean survival time of 16 – 30 days representing 64.41– 89.71% inhibition of parasitaemia in the curative test. These reductions were statistically significant (P<0.05) comparable to that of the standard drug used (Chloroquine and Pyrimethamine). These results show that the aqueous leaf extract of M. pruriens possesses significant (P<0.05) antiplasmodial activity which confirms its use in folkloric medicine in the treatment of fever and other malaria-related disease.
ABSTRACT
Sequential alterations of body weights as well as total lipids, triglycerides, cholesterol and ketone body levels in the blood of Protopterus annectens during twelve month duration of aestivation were investigated. The results revealed that after the first trimester of dormancy, there was significant body weight reduction (p<0.05) coupled with significant hypolipodaemia, hypotriacylgly-cerolaemia and hypocholesterolaemia respectively (p<0.05) but without significant ketonaemia (p<0.05). The total lipid, triglyceride, cholesterol and body weight reductions continued through the second, third and fourth trimesters of aestivation respectively (p<0.05) but with serum ketone body levels remaining unaltered (p<0.05). Thus, the utilization of lipids as a source of energy during aestivation of P. annectens does not lead to ketone body accumulation.
ABSTRACT
Specimens of the west African lungfish, Protopterus annectens (mean weight 294.3 g mean length 38.1 cm), procured from Agulu lake at Agulu in Anambra state, Nigeria were removed from the ambient water and exposed to complete aerial life for a period of seven consecutive days after which some of their haematological changes were determined. There were significant polycythaemia, leucocytosis, thrombocythaemia and elevation of haemoglobin contents (p<0.05). The importance of these haematological adjustments which the fish makes whilst subjected to complete aerial habitat prior to aestivation are discussed.
ABSTRACT
Some specific haematological changes that accompany chronic and severe typhoid fever were investigated in an endemic area of Enugu Urban-Nigeria. The results established that typhoid fever infections led to a statistically significant leucopenia (p < 0.05). In acute, chronic cases, leucopenia is accompanied with significant oligocythaemia, thrombocytopenia, anaemia and lowered haematocrit (p < 0.05). The importance of the results in the diagnosis and treatment of typhoid fever are discussed.